Fig. 6.

Loss of GCN2 decreases cell survival to osmotic stress. A: GCN2+/+ and −/− MEF cells were exposed to 200, 300, and 400 mM urea for 16 h. Cells were incubated with WST-1 to evaluate cell survival. The percentage of viable cells was calculated as control cells were defined as 100% viable. *Significant difference between GCN2+/+ and GCN−/− cells (t-test; P < 0.05, n = 7). B: Western blot analysis of activated caspase-3 levels in whole cell lysates from GCN2+/+ and GCN−/− MEF cells after treatment with 200, 300, and 400 mM urea for 6 h.