Fig. 4.

CC does not alter cell ATP levels in BU.MPT cells subjected to metabolic stress. ATP levels were measured (nM ATP/mg cell protein). ATP levels in cells subjected to metabolic stress were expressed as a percentage of ATP levels under control conditions (defined as 10 mM dextrose, without CC). A: ATP levels in BU.MPT cells were measured after incubation for 8 h in dextrose-containing (dark gray bars) or dextrose-free (light gray bars) medium containing varying concentrations of CC. B: ATP levels were measured in BU.MPT cells after incubation for 8 h in dextrose-free medium containing varying concentrations of DOG in the absence (dark gray bars) or the presence (light gray bars) of CC (20 μM). *P < 0.001, DOG at all concentrations, in both the presence and the absence of CC vs. control unstressed cells. #P < 0.01, each DOG concentration vs. preceding DOG concentration, in both the absence and the presence of CC (20 μM). C: BUMPT cells were incubated with antimycin A (2 μM) and varying concentrations of dextrose in the absence (dark gray bars) or the presence (light gray bars) of CC (20 μM) for 8 h, after which ATP levels were measured. *P < 0.001, antimycin A vs. control unstressed cells, at all dextrose concentrations, in both the presence and the absence of CC (20 μM). #P < 0.01, each dextrose concentration vs. preceding dextrose concentration in both the presence and the absence of CC.