Fig. 1.

The infarct volume increased with the duration of middle cerebral artery occlusion (MCAO). The brain slices were subjected to triphenyltetrazolium chloride (TTC) staining at 24 h after reperfusion following MCAO and the ischemic infarct volume was quantified by a computerized image system. The slices on the right of each column show the backside of the left slices. Note that the infarct regions (pale white portion) were mainly located in the striatum and frontoparietal cortex in the right hemisphere and the volume of the ischemic infarction was largely enlarged when MCAO was extended from 30 min (A) to 60 min (C). After 60 min of MCAO, the infarction volume was >1/3 of the whole brains.