Table 1.
Effect of different temperatures during egg incubation and the first-feeding periods on weight and expressions of trypsin and chymotrypsin (expressed as lmol p-nitroaniline h–1 mg protein–1) and on the activity ratio of trypsin to chymotrypsin (T/C ratio) during the first winter in Atlantic salmon parr possessing different trypsin phenotypes
| Temperature (°C) | Parameter (mean±sem) | Trypsin isozyme pattern | ||
|---|---|---|---|---|
| 1 | 2 | 3′ | ||
| Hatching | Number | 24 | 25 | 18 |
| 5.9±1.9 | Weight (g) | 14.6±0.5 | 15.6±0.4 | 15.4±0.5 |
| Start-feeding | Trypsin | 68.5±3.7 | 72.4±4.2 | 80.1±5.7 |
| 5.6±1.3 | Chymotrypsin | 7.9±0.6a | 7.5±0.5a | 9.6±0.7b |
| (Cold-Cold) | T/C ratio | 9.9±1.0 | 10.7±0.9 | 9.3±1.1 |
| Hatching | Number | 7 | 28 | 23 |
| 5.9±1.9 | Weight (g) | 34.5±2.3 | 33.0±1.0 | 34.8±1.4 |
| Start-feeding | Trypsin | 73.5±6.9 | 77.6±3.4 | 72.5±3.7 |
| 12.2±0.5 | Chymotrypsin | 3.5±0.3 | 4.1±0.2 | 4.4±0.2 |
| (Cold-Warm) | T/C ratio | 22.4±3.3 | 20.2±1.4 | 17.4±1.3 |
| Hatching | Number | 32 | 21 | 17 |
| 9.6±1.2 | Weight (g) | 17.5±0.7 | 19.9±1.0 | 16.1±1.0 |
| Start-feeding | Trypsin | 72.3±3.6 | 68.3±3.6 | 73.4±5.3 |
| 5.6±1.3 | Chymotrypsin | 9.1±0.7 | 9.3±0.4 | 8.7±1.0 |
| (Warm-Cold) | T/C ratio | 9.5±0.9 | 7.8±0.6 | 11.7±2.1 |
| Hatching | Number | 11 | 29 | 23 |
| 9.6±1.2 | Weight (g) | 46.3±4.5a | 66.3±3.7b | 61.2±4.8b |
| Start-feeding | Trypsin | 81.9±5.7 | 85.8±4.8 | 70.9±4.5 |
| 12.2±0.5 | Chymotrypsin | 4.9±0.4 | 4.9±0.2 | 4.9±0.3 |
| (Warm-Warm) | T/C ratio | 17.8±1.7 | 18.4±1.4 | 15.8±1.7 |
The values with different superscripts are significantly different (p<0.05). See details of each trypsin pattern in the text and in the legend of Figure 2.