Figure 6.

SLO-1 was mislocalized in both body-wall muscle cells and neurons of dyb-1 mutant. A, SLO-1::GFP in the dorsal cord and ventral cord appeared as puncta in the wild type but was diffusely distributed in dyb-1 mutant. B, SLO-1::GFP in muscle was localized to dense body regions in the wild type but undetectable in dyb-1 mutant. C, D, The presynaptic marker RIM (C) and dense body marker vinculin (D) were normally localized in dyb-1 mutant. E, Quantification of the density of dorsal cord puncta shown in A and C. *p < 0.0001, significantly different from the wild type (unpaired t test). F, Western blot shows that the level of SLO-1::GFP total protein was comparable between the wild-type and dyb-1 mutant. α-Tubulin was blotted to show equal loading of the protein samples. Scale bars, 10 μm.