Fig. 4.

Dose response of H₂O₂ on the frequency of rhythmogenesis from the preBötC. A: representative plots of the running average of f_inst as a function of time when exposed to 440 μM (i), 1.1 mM (ii), 2.2 mM (iii), or 4.4 mM (iv) H₂O₂. Using the plots of the running average of f_inst revealed that H₂O₂ caused an initial transient concentration-dependent suppression in population activity from the preBötC. B, left: plotting the duration of the suppressed f_inst as a function concentration revealed the H₂O₂-mediated transient suppression of f_inst at 440 μM (n = 4/6), 1.1 mM (n = 3/5), 2.2 mM (n = 17/20), and 4.4 mM (n = 6/6) H₂O₂. Right: plotting the mean normalized f_inst values for the experiments showing an transient suppression of f_inst illustrates the biphasic nature of the frequency response of rhythmogenesis to H₂O₂ and the ability of H₂O₂ to augment frequency at the highest concentrations tested.