Fig. 9.

Effects of Fe²⁺-H₂O₂ coapplication on K⁺-mediated population bursting in the CA1 neuronal population of the hippocampus. A: representative trace of the integrated population activity (∫ CA1) during control, supplementation of Fe²⁺, and addition of H₂O₂ (2.2 mM) in Fe²⁺-supplemented aCSF. Addition of H₂O₂ in the presence of Fe²⁺ yields ^•OH via Fenton chemistry. B–D: the effects of Fe²⁺-H₂O₂ on burst frequency (B), irregularity score of f_inst (C), and integrated burst area (D) were determined by comparing a 120-s bin during control (CON) to bins ending at the elapsed times of 180, 300, 600 (Fe²⁺; light gray bars) and 780, 900, and 1,200 (Fe²⁺-H₂O₂; dark gray bars). #Irregularity score of f_inst was not reported for time bin = 1,200 because ≤1 burst occurred during this bin window in 2 of 4 individual experiments. n = 4. **P < 0.01.