Fig. 6.
Controls for bundle integrity. Normalized bundle stiffness (kB/kmean, where kmean is the mean of 4 trials and each trial is plotted in the figure) as a function of time for 2 control conditions. A: bundle stiffness declines over time in the presence of subtilisin (●; n = 4). In contrast, bundle stiffness in a control solution (the same solution minus subtilisin; ▴; n = 2) is stable for up to 5 h. B: bundle stiffness declines precipitously in BAPTA (●; n = 4). Lines in A and B are Loess fits, which summarize trends in the data.