Table 1.
Single-cell RT-PCR primer pairs for rat neuronal Nav channels, TH, and GAD1 mRNAs
| mRNA (Accession No.) | Start Position | Primers (5′ to 3′) | |
|---|---|---|---|
| Nav1.1 (NM_030875) | F | 4659 | ggctcgttcttcactctaaa |
| R | 5106 | ttcctacaatggagaggatg | |
| Nav1.2 (NM_012647) | F | 1641 | aggcgggataggtgttttct |
| R | 1991 | gcaaagtcattttcggaacc | |
| Nav1.3 (NM_013119) | F | 3291 | ggaaggatgcaaaagggaat |
| R | 3586 | acagcaattggcacagtcac | |
| Nav1.6 (NM_019266) | F | 2808 | ggccatcatcgtcttcatct |
| R | 3058 | ggttgccaatgaccataacc | |
| Navβ1 (NM_017288) | F | 820 | ctggccattacttccgagag |
| R | 1118 | gccatattgcttcacccatc | |
| Navβ2 (NM_012877) | F | 403 | gttcctccagttccgaatga |
| R | 751 | gtcatccgtgctcagcttct | |
| Navβ3 (NM_139097) | F | 1850 | ctgggccagagatgactttc |
| R | 2149 | acacgcccatctttgtctct | |
| Navβ4 (AF544988.1) | F | 380 | cccaaactgttctttctgag |
| R | 729 | caatcccagagttctagtgc | |
| TH (NM_012740) | F | 1130 | cactgtggaattcgggctat |
| R | 1329 | cattgaagctctcggacaca | |
| GAD (NM_017007) | F | 1567 | caagttctggctgatgtgga |
| R | 1826 | actccatcatcagggctttg |
Nav, voltage-activated sodium; TH, tyrosine hydroxylase; GAD, glutamate decarboxylase; F, forward; R, reverse.