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. 2011 Aug 31;106(6):2922–2935. doi: 10.1152/jn.00634.2011

Fig. 6.

Fig. 6.

Effects of Ca2+ buffering on release efficiency. A, C, and E: cone ICa evoked by a step from −70 mV to −10 mV (100 ms) overlaid on the simultaneously recorded horizontal cell EPSC. B, D, and F: number of vesicle fusion events per channel opening obtained by deconvolution of the EPSC and ICa, respectively. In A and B, the cone pipette solution contained 0.5 mM EGTA. In C and D, the cone pipette solution contained 1 mM BAPTA. In E and F, we used a gramicidin-perforated patch recording technique to maintain endogenous Ca2+ buffering. G: average peak efficiency of release obtained when using a step depolarization to −10 mV and cone Ca2+ buffering provided by 5 mM EGTA (n = 18), 0.5 mM EGTA (n = 6), 1 mM BAPTA (n = 6), or endogenous Ca2+ buffers (perforated patch, n = 5). H: average peak efficiency of release with these same buffers obtained with a ramp voltage protocol (0.5 mV/ms; 5 mM EGTA, n = 9; 0.5 mM EGTA, n = 6; 1 mM BAPTA, n = 6; perforated patch, n = 5).