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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Eur J Immunol. 2011 Aug 31;41(10):3006–3016. doi: 10.1002/eji.201141606

Figure 1.

Figure 1

B and T cells express Camp and mCRAMP. MZ B cells, FO B cells, CD4+ and CD8+ T cells were purified from C57BL/6 spleens while B1a, B1b, B2, and total T cells were purified from peritoneal cavity washes. Total spleens and peritoneal cavity washes were also collected from Camp−/− (KO) mice. (A) Total RNA was isolated and analyzed using RT-PCR analysis for the mRNA levels of Camp, CD19, CD3e, and actin. (B) Total protein was isolated from synthetic mature mCRAMP, sort-purified CD43− B cells, CD4+CD62L+ naïve T cells, Camp−/− spleen, and neutrophils and analyzed by Western blot for the expression of mCRAMP and β-actin. (C) B and T cells were activated in vitro with the indicated stimuli and the level of Camp and actin mRNA was measured by real-time PCR and presented as fold change in Camp expression normalized to actin. Data represent the mean1SEM from three independent experiments. One representative gel and blot are shown. Data were analyzed by a one-way ANOVA followed by post hoc analysis. *p<0.05, *p<0.01.