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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Eur J Immunol. 2011 Aug 31;41(10):3006–3016. doi: 10.1002/eji.201141606

Figure 2.

Figure 2

Camp-deficient T cells produce more IL-4 in vitro. Purified naïve CD4+CD62L+ T cells were cultured under Th1 (anti-CD3, -CD28, rIFN-γ)or Th2 (anti-CD3, -CD28, -IL-12, and rIL-4) driving conditions, in the presence or absence of mCRAMP. Total RNA was collected from WT (black bars) and Camp KO (white bars) cultures on day 4 and analyzed by RT-PCR for (A) IFN-γ and (D) IL-4. T cells were also collected and stained for intracellular (B and C) IFN-γ and (E and F) IL-4. (B and E) The total number and (C and F) fold change in MFI of IFN-γ and IL-4 are shown. Data represent the mean+ SEM from three independent experiments. Data were analyzed by a one-way ANOVA followed by post hoc analysis. *p<0.05, *p<0.01.