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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Eur J Immunol. 2011 Aug 31;41(10):3006–3016. doi: 10.1002/eji.201141606

Figure 6.

Figure 6

Camp-deficient mice produce more IL-4 and induce more antigen-specific IgG11 ASCs. Total splenic cells were isolated on days 7 and 4 following primary and secondary immunization with TNP-OVA/alum, respectively. (A) ELISpot analysis was performed on the splenic cells for anti-TNP-OVA IgG1 ASCs. (B) Total RNA was isolated and RTPCR analysis was performed for the level of IL-4 and actin mRNA, shown as fold change in IL-4 expression normalized to actin. (C) Flow cytometric analysis was performed and total CD41IL-41 T cells were determined by intracellular cytokine staining. Data represent the mean 1SEM from three independent experiments, five mice per group. Data were analyzed by a two-tailed unpaired t test. *p<0.05.