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Binding mechanism of N-alkylamine derivatives. A. Saturation binding of [3H]-(+)-pentazocine in the presence of increasing concentration of 2b. Data points were obtained in duplicates and the non-specific binding (in the presence of 10 μM haloperidol) was subtracted from the total binding (without haloperidol). B. Scatchard transformation of the binding curves shown in B. C. Bmax and KD values of [3H]-(+)-pentazocine in the presence of 20 nM and 40 nM of 2b.
