Figure 2. Wolbachia-induced CI phenotype in G. m. morsitans.

Normal reproduction between Gmm^Wt females and males is discernible by a developing oocyte indicated by the white arrow and the presence of a larva in the uterus indicated by the pink arrow, following the first gonotrophic cycle. (B) Unmated adult female tsetse. Unmated Gmm^Wt females have an empty uterus and multiple developing oocytes indicated by white arrows. Note the transparent nature of the spermatheca reflective of lack of sperm (C) Manifested CI. CI is indicated by Gmm^Apo females mated with Gmm^Wt males by the absence of a larva in the uterus and deformed embryo indicated by the blue arrow. Many of these embryos were aborted without hatching into larva. Orange arrows indicate spermathecae in each image. Images were collected forty days (corresponding to the second gonotrophic cycle) post mating.