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. 2011 Dec 8;7(12):e1002422. doi: 10.1371/journal.ppat.1002422

Figure 5. Co-localisation and physical interaction of IRGM with MeV-C, HCV-NS3 and HIV-NEF proteins.

Figure 5

(A) IRGM co-localises with MeV-C, HCV-NS3 and HIV-NEF. GFP-IRGM was expressed in HeLa cells together with FLAG-MeV-C or FLAG-HCV-NS3 or FLAG-HIV-NEF. Co-localisation between GFP-IRGM and FLAG-tagged proteins was visualized as in figure 2B. Scale bars, 5 µM. (B) IRGM physically interacts with MeV-C, HCV-NS3 and HIV-NEF. HEK-293T cells were co-transfected with expression vectors encoding FLAG-IRGM and GST alone or GST fused to the indicated viral proteins. Co-purification of IRGM with viral proteins was assessed as in figure 2C. One experiment representative of two is shown.