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. 2011 Dec 8;7(12):e1002433. doi: 10.1371/journal.ppat.1002433

Figure 7. Characterization of nsp1-induced RNA fragment of ALA mRNA.

Figure 7

Cap-labeled ALA mRNA was incubated with GST, nsp1 or nsp1-mt in RRL. RNAs were extracted from RRL by using proteinase K digestion and subsequent phenol/chloroform extraction. A cap-radiolabeled RNA fragment was detected in a 10% polyacrylamide/7M Urea DNA sequencing gel electrophoresis. An RNA size marker (ranging from 10 nt to 100 nt in 10 nt increment) was prepared by using the Decade Marker system (Applied Biosystems) and applied to the same gel.