Figure 4. Hydrolysis of basement membrane components in vitro.

Hydrolysis of laminin (A), nidogen (B), type IV collagen (C) and perlecan (D) by BaP1 and leuc-a, as detected by Western blotting of Matrigel. Matrigel was incubated with either BaP1, leuc-a or PBS (control, lane C), as described in the legend of Fig 3. Matrigel preparations were separated by SDS-PAGE under reducing conditions using a 4-15% gradient gel and transferred to nitrocellulose membranes. Immunodetection was performed with either anti-laminin, anti-nidogen, anti-type IV collagen or anti-perlecan antibodies. Reaction was developed with a chemiluminiscent substrate.