Table 1. Mol. mass of most abundant fragments of BM proteins generated by BaP1 and Leuc-a.
Matrigel in vitro | In vivo | |||
Protein | Leuc-a | BaP1 | Leuc-a | BaP1 |
Type IV Collagen | 140, 120 | 155, 140, 100 | -a | 110 |
Laminin | 150, 95, 60 | 150, 95, 60 | -b | 285 |
Nidogen | 95, 50 | 95, 50, 40 | 100, 50 | 100, 50 |
Perlecan | 170, 120, 40,30 | 170, 120, 35, 30 | -c | 230 |
No degradation bands were detected by immunoblotting, and the intensity of the main bands of type IV collagen was not reduced.
No degradation bands were detected by immunoblotting, but the main laminin bands were reduced as compared to control samples.
No degradation bands were detected by immunoblotting.