Table 1.

Summary of mutations and expression for selected synthetic promoters

Promoter naming convention: CM, CMV promoter mutant; EF, EF1α promoter mutant; C, T, or R—CAAT, TATA, or random mutagenesis, respectively. The TATA and CAAT box sequences of the wild-type promoters (CMVwt or EF1αwt) are in bold, and the transcription start site (TSS) is underlined. Base position is given in parentheses at the ends of the sequence and corresponds to the distance from the TSS. Mutations listed under the wild-type sequences: –, no mutation; Δ, base deletion. GFP expression values from HEK-293 cells are arithmetic means ± s.d. (n = 3) calculated from geometric means of each sample population