Figure 3.

Quantitative RT-PCR Analysis of Kif22 Expression in Wild-Type Mouse Growth Plate Cartilage Zones

qPCR was performed for Kif22 on cDNA derived from proliferative and hypertrophic zones microdissected from 2-week-old wild-type mouse tibial growth plates (n = 3). qPCR was conducted with three technical replicates. Kif22 expression was calculated relative to the housekeeper gene Mrps16 and expressed as the fold difference in expression in the proliferative zone compared to the hypertrophic zone. Statistical significance was calculated with the Student's t test. Error bars represent standard deviation. Detailed quantitative expression data are reported in Table S1.