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. 2011 Dec 9;89(6):773–781. doi: 10.1016/j.ajhg.2011.11.002

Figure 4.

Figure 4

smoc2 Morphants Exhibit Tooth-Germ Defects

(A–I) In situ hybridization of both dlx2b (A–D; probe obtained from D.W. Stock, Colorado, USA), bmp2a (E and F; probe obtained from M. Hammerschmidt, Cologne, Germany) and pitx2 (G–I, pitx2 full-length cDNA was cloned into the Flc3 plasmid [Riken]) on wild-type (A, E, and G), smoc2-2 (B, F, and H), smoc2-1 (D and I), and smoc2-1cont morphants (C) shows a loss of dlx2b and bmp2 expression in smoc2 morphants (B, F, and D) and reduced and fused expression of pitx2 (H and I). The smoc2-1cont morphants do not show any defects.

The arrows represent teeth germs; the arrow heads represent missing dlx2b or bmp2a expression. The black star represents a gap within pitx2 expression, the white star represents fused pitx2 expression. Abbreviations are as follows: pf, pectoral fin; tg, teeth germs; fb, forebrain; and pp, pharyngeal pouches. Lateral (C and D) and ventral (A, B, E–I) views of embryos, 56 hpf. The scale bar represents 100 μm.