Figure 4.

Localization of Group V Phospholipase A₂ within a Control Human Retinal Tissue

Human retinal tissue from an 87-year-old male donor's eye was obtained from the eye bank at Moorfields Eye Hospital with the approval of Moorfields and Whittington Research Ethics Committee (06/Q0504/78) and embedded in an optimal-cutting-temperature compound. Cryostat sections were cut at 10 μm and thaw-mounted onto charged slides. Immunohistochemistry was performed at room temperature to reveal group V phospholipase A₂ localization via mouse anti-human PLA2G5 monoclonal antibody (LS-C11702, clone MCL-3G1, Lifespan Bioscience, Seattle, WA, USA)³⁰ at a dilution factor of 1/20. An alkaline phosphatase-conjugated avidin-biotin complex kit (Vectastain ABC-AP Mouse IgG kit, Vector Laboratories, Burlingame, CA, USA) was used as a secondary detection method according to the manufacturer's guidelines. An additional quenching step was performed with 1% Levamisole for 30 min so that autofluorescence would be reduced.

Abbreviations are as follows: Ch, Choroid; RPE, retinal pigment epithelium; OS, photoreceptor outer segments; IS photoreceptor inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; an dG ganglion cell layer. The scale bar represents 50 μm.