Related to Figure 5
(A) RLuc/FLuc mRNA ratio measured by qRT-PCR in C2 myoblasts transfected with RLuc-MD1-WT construct together with plasmids expressing miR-133a/b, miR-135a/b or control. Values are normalized for HPRT and shown with respect to pCtrl set to a value of 1.
(B) The constructs described in Figure 5 were cotransfected in C2 myoblasts with scamble LNA (LNA-scr) or LNA for the indicated miRNA. Histograms indicate the values of renilla luciferase measured 48 hr after transfection in DM. Data are shown with respect to the values of RLuc control vector set to a value of 100%. One asterisk: p < 0.05.