Table 1. Percentage of neurons expressing MeCP2 transgenes in selected areas of the brain.
| e1-TgH | e1-TgL | e2-Tg | |
|---|---|---|---|
| Cortex | |||
| Somatosensory (S1; %) | 67±18 | 32±11 (except layer 2: 96±16) | 63±12 |
| Ectorhinal (Ect; %) | 81±19 | 40±14 | 79±13 |
| Entorhinal (MEnt; %) | 84±16 | 76±26 | 82±16 |
| Striatum (CPu; %) | 72±12 | 27±11 | 77±18 |
| Hipoccampus | |||
| CA1 (%) | 16±8 | 23±11 | 86±14 |
| CA2 (%) | 86±15 | 19±14 | 88±9 |
| CA3 (%) | 85±19 | 16±9 | 76±19 |
| DG (%) | 92±22 | 22±13 | 9±5 |
| Inferior colliculus (%) | 96±17 | 21±8 | 88±7 |
| Cerebellum (Crus1; %) | 98±3 | 21±8 (2% in the molecular layer) | 77±12 |
| Thalamus (LPMR+AVDM; %) | 64±10 | 7±7 | 82±16 |
| Brain stem | |||
| Oral pontine reticular nucleus (%) | 66±15 | 51±12 | 60±16 |
| Intermediate reticular nucleus (%) | 42±11 | 46±9 | 32±14 |
| Inferior olive (%) | 68±19 | 31±16 | 79±22 |
Abbreviations: MeCP2, methyl-CpG binding protein 2; S1, primary somatosensory cortex; Ect, ectorhinal cortex; MEnt, entorhinal cortex, medial part; CPu, caudate/putamen; Crus1, superior semilunar lobule; LPMR, lateral posterior nucleus; AVDM, anteroventral nucleus, according to MBL (Mouse Brain Atlas Nomenclatures).
Brain sections of 40 μm obtained from Mecp2+/Y;e1-TgH, Mecp2+/Y;e1-TgL and Mecp2+/Y;e2-Tg mice of 4 weeks of age were stained for anti-MeCP2 and anti-myc or anti-EGFP. The percentage of cells expressing tagged versions of MeCP2 was calculated as cells positive for anti-myc or anti-GFP/cell expressing MeCP2 × 100. At least 50 cells were counted per region per animal (n=3). The specific area from which cell counting was performed is indicated between brackets.