Fig. (4).

Comparison of different reporter formats and promoters. The effect of the different promoter use in the full-length AR (A) and GR (B) reporter assays was analyzed by correlating LogEC₅₀ and LogIC₅₀ values of compounds active in the reporter assays containing GRE in the reporter promoter and the same values obtained from reporters with MMTV promoter. Pearson correlation coefficient was calculated: AR (Pearson r coefficient = 0.95, r² = 0.89, P < 0.0001), GR (Pearson r coefficient = 0.88, r² = 0.77, P < 0.0001). Correlation of compound potencies obtained from different reporter formats. LogEC₅₀ together with LogIC50 values of compounds active in the ERα (C), ERβ (D) and GR (E) full-length reporter assays were plotted against the same values obtained from Gal4/LBD reporter assays. Pearson correlation coefficient was calculated: ERα (Pearson r coefficient = 0.94, r² = 0.89, P < 0.0001), ERβ (Pearson r coefficient = 0.91, r² = 0.82, P < 0.0001), GR (Pearson r coefficient = 0.80, r² = 0.63, P =0.0034).