FIG. 3.

Prx3 deficiency results in increased adipogenic gene expression during adipogenesis. (A) Adipose tissue-derived stem cells (ASCs) were isolated from subcutaneous fat pads of WT and Prx3 KO mice and differentiated with or without rosiglitazone. Differentiated cells were stained with Oil Red O and representative pictures were taken. (B) Genes related to adipogenesis and mitochondrial biogenesis were examined in ASCs from WT and Prx3 KO mice by real-time PCR. Quantifications were normalized to 18S in each reaction. Values are means±SE of six experiments. *p<0.05 vs. WT ASCs. (C) Transfection efficiency was validated by reduced Prx3 expression at day 4 after differentiation. There was no change in Prx1 or Prx5 levels by Prx3 siRNA transfection. Western blot analysis of 3T3-L1 cells transfected with (D) Prx3 siRNA or (E) Prx3 vector. 3T3-L1 cells were differentiated 48 h after transfection. Cells were harvested at days 0, 2, and 4 after differentiation. Scrambled (Scr) siRNA or empty vector was used as transfection control. Tubulin was used as an internal loading control. (To see this illustration in color, the reader is referred to the web version of this article at www.liebertonline.com/ars).