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STAT4 mRNA levels and half-life of STAT4 mRNA in PBMCs. PBMCs obtained from 6 healthy control subjects and 3 patients after high-dose chemotherapy and PBSCT were treated with actinomycin D at 1 μg/mL for 0, 2, 4, and 6 hours in a 5% CO2 incubator at 37°C. RNA was extracted and first-strand cDNA was synthesized, followed by the real-time PCR.25 The half-life of STAT4 mRNA from each sample was calculated accordingly.25 Results shown are means ± SD.
