Figure 4.

Fewer colon TS1 cells accumulate in recipients of T_EM than in recipients of T_N or T_CM. HA104 mice received 750cGy and 8 × 10⁶ RAG^−/− BM cells in combination with 3000 TS1 T_N, T_CM, or T_EM. On days 7, 14, 21, and 28, mice were killed and spleen, liver, BM, and colon cells were isolated. In addition, skin, colon, and liver samples were taken for analysis of histopathology. Representative FACS plots of CD4 and TS1 staining in the spleen, liver, BM and colon of T_N, T_CM, and T_EM recipients 14 days after transplantation are shown in (A). Total numbers of TS1 in spleen, liver, BM and colons of T_N, T_CM and T_EM recipients are shown in (B). In the colon, P values comparing the number of TS1 cells in T_N versus T_EM recipients were as follows: day 7, P = .0021; day 14, P = .0015; day 21, P < .0001; day 28, P = .0286. Skin, liver and colon pathology scores are shown in (C). For day 21 colon scores, P = .0025 comparing scores in recipients of T_EM to the combined scores from T_N and T_CM recipients. Results shown are combined from 2 independent experiments. In the first experiment, a minimum of 4 mice per group were killed on days 7,14, 21, and 28. In the second experiment a minimum of 4 mice per group were killed on days 7, 14, and 21. Day 21 pathology scores for T_N recipients are also shown in Figure 1D.