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PulS and Sdom form a stable 1:1 complex by size exclusion chromatography and analytical ultracentrifugation. A, size exclusion chromatography elution profiles of 40 μm PulS, 40 μm Sdom, and an equimolar ratio of PulS and Sdom, preformed at 20 °C. B, continuous sedimentation coefficient distribution analysis of 100 μm Sdom, 100 μm PulS, and PulS/Sdom mixture (75 μm Sdom, 75 μm PulS). Sedimentation coefficients are expressed in Svedberg units (1 S = 10−13 s).
