TABLE 1.

Hydrodynamic properties of PulS, Sdom, and the PulS-Sdom complex

	PulS	Sdom	PulS-Sdom
Molecular massa	NDb	9.7 ± 0.1 kDa	22.1 ± 0.7 kDa
Sedimentation coefficient (10−13 s)c	1.4 ± 0.1	1.1 ± 0.1	1.9 ± 0.1
RH (nm)c	1.7	2.5	2.7
Frictional ratio (f/f0)d	1.2	1.9	1.5
Intrinsic viscosity (ml·g−1)a	NDb	16.9 ± 0.7	5.9 ± 0.1
Viscosity increment, νe	NDb	4.4	2.5

^a Data were determined by static light scattering and viscometry experiments using size exclusion chromatography-coupled triple detector array.

^b ND means not determined.

^c Data were determined by analytical ultracentrifugation experiments.

^d Data were calculated from the experimental hydrodynamic radius and the hydrodynamic radius of an anhydrous and spherical molecule with equivalent molecular mass and partial specific volume.

^e Data were calculated using Einstein's viscosity relation, see under “Experimental Procedures.”