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Identification of the hydroxyls involved in the binding of the SadP adhesin to Galα1–4Gal. A, microtiter wells were coated with 1 μg/ml pigeon ovomucoid, and inhibition studies were performed by incubating 5 μg/ml SadP adhesin in the presence of 50 mm d-glucose, d-galactose, or N-acetylgalactosamine or a 500 μm concentration of the O-deoxy derivatives of Galα1–4Gal as indicated. B, schematic diagram of the binding mechanism of S. suis SadP adhesin to Galα1–4Gal. Error bars, S.E.
