FIGURE 4.

NFκB binds to the fascin promoter in response to OSM in a STAT3-dependent manner. A, 4T1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS and treated with OSM for 30 min. ChIP assays were performed with an NFκB antibody and primers flanking the potential NFκB binding site. B, results from A were quantitated with a PhosphorImager and expressed as ChIP/Input with the untreated sample set at 1. C, 4T1 cells were transfected with either a control siRNA or both STAT3 siRNAs (1+2) together and cultured for 3 days. Cells were treated with OSM for 30 min and ChIP assays were performed with STAT3 or NFκB antibodies and primers flanking the potential STAT3/NFκB binding site.