FIGURE 5.

NFκB is required for cytokine-induced fascin expression. A, MDA-MB-231 cells were transfected with either a control siRNA or two NFκB p50 siRNAs either together (1+2) or separately (1 or 2) and cultured for 4 days. Whole cell extracts were performed, and Western blot analyses were performed with antibodies against NFκB p50 or tubulin. B, portions of the cells in A were treated with IL-6 and IL-6R for 30 min, and RNA was subjected to quantitative real-time RT-PCR for fascin and GAPDH. Results were standardized to GAPDH and expressed as -fold induction with untreated samples set as 1.