FIGURE 6.

Hindrance of the Arl4A-ELMO1 complex reduces stress fiber disassembly. A, coexpression of Myc-ELMO1^WT and Arl4A^WT reduced F-actin intensity, indicating stress fiber disassembly, whereas coexpression of Myc-ELMO1^L43A and Arl4A^WT did not induce substantial stress fiber disassembly. Quantification of average fluorescence intensity of F-actin in cells expressing the indicated plasmids. Student's t test was performed to compare each condition (**, p < 0.01). B, hindrance of the Arl4A-ELMO complex reduces stress fiber disassembly. There are three different filamentous actin morphologies: (i) normal (long thick actin fibers), (ii) thin (thinner but ordered actin fibers), and (iii) disrupted (disordered thin actin fibers). Quantification of cells with different actin morphologies is shown. HeLa cells transfected with the indicated plasmids were fixed, and filamentous actin was visualized with FITC-phalloidin. ARL4A-expressing cells show thin-to-disrupted actin morphologies, with coexpression of ELMO1^WT but not ELMO1^L43A increasing the percentage of cells exhibiting disrupted actin morphology. For each condition, more than 60 cells were analyzed.