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AES interacts with the FG repeat region of NUP98-HOXA9. A, full-length NUP98, NUP98-HOXA9, and NUP98-HOXA9 deletion mutants. Vertical lines represent FG repeats. DBD, DNA-binding domain. B, GST-AES or GST were immobilized on beads and incubated with in vitro translated full-length NUP98, NUP98-HOXA9, or deletion mutants of NUP98-HOXA9. Bound and unbound fractions were resolved by SDS-PAGE and subjected to autoradiography.
