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. 2011 Sep 21;286(45):38989–39001. doi: 10.1074/jbc.M111.297952

FIGURE 6.

FIGURE 6.

AES cooperates with NUP98-HOXA9 in transcriptional activation. K562 cells were transfected with a luciferase construct driven by the KBTBD10 promoter in addition to either empty vector or vector expressing NUP98-HOXA9, without or with AES. Firefly luciferase activity was measured 48 h after transfection and normalized to a Renilla luciferase internal control. The numbers represent fold-change over control (average of three independent experiments); error bars represent S.D. The p value indicated was obtained by a two-tailed t test. Bottom panel, expression of HA-NUP98-HOXA9 and AES was verified by immunoblotting with anti-HA and anti-AES antibody, respectively.