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. 2011 Sep 21;286(45):38989–39001. doi: 10.1074/jbc.M111.297952

FIGURE 7.

FIGURE 7.

shRNA-mediated knockdown of AES inhibits downstream gene regulation by NUP98-HOXA9. K562 cells were transfected with a firefly luciferase construct driven by the KBTBD10 promoter with either empty vector or vector expressing NUP98-HOXA9. In addition, the transfections included empty vector, vector expressing nonspecific shRNA, or 2 vectors expressing different AES shRNAs. Firefly luciferase activity was measured 48 h after transfection and normalized to a Renilla luciferase internal control. The numbers represent fold-change over control (average of three independent experiments); error bars represent S.D. The p value indicated was obtained by a two-tailed t test. Expression of HA-NUP98-HOXA9 and knockdown of AES were verified by immunoblotting with anti-HA or anti-AES antibody, respectively.