TABLE 2.

Parameter values for CaM binding to GST fusion peptides

Data from the GST pull-down assay shown in Figs. 3 and 4 were analyzed based on Equation 5 by Kell Ligand software. ND, no data.

GST fusion peptide	Bmax	Kd
		Without CSL	With CSL
	mol/mol	μm
2+
NT	1.01	0.42	1.1
LI-II	0.34	0.54	ND
CT1	1.14/0.8a	0.23/17.84b	1.17/29.12b
IQ	0.76	1.72	5.81
In 80 nm Ca2+
NT	0.28	4.58	ND
LI-II	0.16	4.74	ND
CT1	0.37	0.66	5.22

^a CaM binding to CT1 in 1 mm Ca²⁺ was fitted to a two-site model and expressed as B_max1/B_max2.

^b CaM binding to CT1 in 1 mm Ca²⁺ was fitted to a two-site model and expressed as K_d1/K_d2.