TABLE 1.

Primers

Primer	Sequence (5′-3′)	Recombinant plasmid
hspB	5′-caaccatggcaatgagcatgaaacagcgcgtaat-3′	pET28a-hspB
	5′-cctgcgctcgagacagaaaggtttccatagtctctg-3′
hspB mutation	1: 5′-atgagcatgaaacagcgcgtaattatcg-3′	hspB mutant
	2: 5′-tctcgacgcacgaagctattgattaaccaatccccttcgacaat-3′
	3: 5′-attgtcgaaggggattggttaatcaatagcttcgtgcgtcgaga-3′
	4: 5′-cagaaaggtttccatagtctctggaa-3′
hspB deletion	5′-gcaggaattcgtctgttcgggtagttcttgg-3′	S16-hspB deletion
	5′-gatagtcgacgtaacgcgctggtgcattc-3′
hspA deletion	5′-cagtgaattctcgaccctgaaaatccggac-3′	S16-hspA deletion
	5′-gatagtcgacatggttggtttcctccctg-3′