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. 2011 Sep 20;286(45):39200–39210. doi: 10.1074/jbc.M111.256123

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — SNF2H binding to origins is inhibited by Cdt1 silencing and enhanced by Cdt1 overexpression. A–C, HeLa cells transfected with control (DS scrambled-Neg; siCont) or Cdt1 (siCdt1) siRNAs for 48 h were subjected to immunoblotting (A), FACS analysis (B), or ChIP assay (C). D--F, HEK293T cells transfected with the indicated expression vectors were analyzed by immunoblotting (D), FACS analysis (E), or ChIP assay (F). Coomassie Brilliant Blue (CBB) staining served as the loading control. For ChIP data, the means ± S.D. are shown (n = 3). *, p < 0.05.