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. 2011 Sep 14;286(45):39224–39235. doi: 10.1074/jbc.M111.286351

FIGURE 3.

FIGURE 3.

UV-visible spectral properties of wild-type BsNOS and mutant proteins W66H and W66F in the presence of H4T and l-Arg. The UV-visible spectral properties of the bacterial NOSs do not differ markedly from those of the mammalian NOS oxygenase. From left to right: BsNOS, W66H, and W66F. In W66F the Fe-CO species (449 nm) slowly decays to an uncharacterized species with absorption maximum at 420 nm (inset). A summary of the absorption maxima is given in Table S1.