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Determination of the redox potential of BsNOS wild-type, W66H, and W66F proteins. The fraction of oxidized protein at each redox potential value was fitted to the Nernst equation. The fitted values for the midpoint potentials are: BsNOS, −361 ± 5 mV; W66H, −302 ± 7 mV; and W66F, −427 ± 5 mV.
