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. 2011 Sep 14;286(45):39247–39258. doi: 10.1074/jbc.M111.264598

FIGURE 5.

FIGURE 5.

OSU-53 inhibits IL-6 signaling through activation of AMPK. A, RT-PCR analysis of the concentration-dependent effect of OSU-53 alone (left upper panel) and after 6 h of pretreatment with 10 μm of the AMPK inhibitor compound C (left lower panel; Cpd C) on LPS-stimulated IL-6 mRNA expression in MDA-MB-231 cells in 5% FBS-containing medium. Right panel, ectopic expression of dominant-negative (DN)-AMPK protects MDA-MB-231 cells against OSU-53-mediated suppression of LPS-stimulated IL-6 mRNA levels. B, Western blot analysis of the concentration-dependent effect of OSU-53 alone (left panel) and after 6 h of pretreatment with 10 μm compound C (right panel) on the phosphorylation levels of AMPK and of downstream markers of IL-6 signaling, including Jak2 and Stat3, in LPS-pretreated MDA-MB-231 cells in 5% FBS-containing medium.