FIGURE 3.

Palmitate-induced apoptosis by DR5 is TRAIL-independent. A, total RNA was isolated from Huh-7 cells treated for 8 h with PA at 400 and 800 μm or vehicle (V). TRAIL mRNA was quantified by real time PCR. Fold induction is expressed in relation to internal control 18 S. B, Huh-7 were treated with PA at 800 μm for 16 h or with TRAIL at 10 ng/ml for 6 h in the presence or absence of DR5-Fc protein chimera (250 ng/ml). Vehicle was used as a control, and caspase-3/7 catalytic activity was measured by the fluorogenic assay. All data are expressed as mean ± S.E. for three experiments; *, p < 0.05.