FIGURE 1.
IC constructs and isoforms. A, sequence alignment between fruit fly and rat IC. Top sequence is D. melanogaster (Dm, AF 263371.1), middle and bottom sequences are R. norvegicus IC-1A (IC-1A, NP 062107.1) and IC-2C (IC-2C, AAA 89165.1) isoforms, respectively. Identical residues (*), conserved residues (:), and semi-conservative substitutions (.) are designated below the aligned sequences. Predicted coiled-coil regions for all sequences are shown in boldface. The binding sites for Tctex1 and LC8 are highlighted in light and dark gray, respectively. Regions 1 and 2 of the p150Glued binding interface are designated above the sequence, as are linker 1 (abbreviated L1) and linker 2. B, IC constructs used in this study. Also highlighted are regions of predicted secondary structure (helices as cylinders and β-sheet as arrow) (44), coiled-coils (45) (double black bars, ICC), regions predicted to be disordered (raised black bars), and binding sites of light chains Tctex1 (light gray) and LC8 (dark gray).