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. 2011 Sep 20;286(45):39349–39359. doi: 10.1074/jbc.M111.296277

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Representative ITC data for IC constructs binding to p150^Glued. Isothermal titration calorimetry data with thermograms (top panels) and isotherms (bottom panels) shown for titration of p150_221–509^Glued with IC:1–143 (A), IC:1–87 (B), and IC:1–40 (C). Data were collected at 25 °C in 50 mm sodium phosphate, 50 mm sodium chloride (pH 7.5). For the data collected with titrants IC:1–143 (A) and IC:1–87 (B), solid lines correspond to the nonlinear least squares fit for an A + B AB binding model.