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. 2011 Sep 20;286(45):39360–39369. doi: 10.1074/jbc.M111.278416

FIGURE 3.

FIGURE 3.

Determination of N-terminal amino acid sequence of inhibitory proteins against S. aureus hemolysin production. Because the amino acid sequence of ApoLp-II/I of B. mori was not identified in previous studies (43), we determined that the nucleotide sequence of cDNA encoding ApoLp-II/I (GenBankTM accession number AB640623). B, mori ApoLp-II (710 amino acids) and ApoLp-I (2607 amino acids) have 70.0 and 59.5% identity with that of Manduca sexta (GenBank accession number U57651 (44)), respectively. Sixty N-terminal residues of B. mori ApoLp-II and ApoLp-I were aligned with those of M. sexta by ClustalW (a and b). Identical residues are marked by an asterisk and similar residues are marked by dots below the alignment. Of 10 N-terminal amino acid residues of the 74-kDa protein in Fraction IV, eight (colored in red in a) were determined and matched with the internal sequence of B. mori ApoLp-II. Ten N-terminal amino acid residues of the 220-kDa protein in Fraction IV (colored in red in b) were matched with the internal sequence of B. mori ApoLp-I. LLT, large lipid transfer domain; VWD, von Willebrand factor module D domain (45).