FIGURE 5.

Intramitochondrial heme accumulation and mitochondrial ferritin expression in gastric mucosal cells after administration of indomethacin. A, intramitochondrial heme content at different time points after indomethacin treatment. Indomethacin-treated rats were sacrificed at different time points, gastric mucosal mitochondria were isolated, and heme was quantitated as described under “Experimental Procedures.” 0 h represents control. B, H₂O₂ damages heme. The fall of Soret spectra at 403 nm with increasing concentrations of H₂O₂ indicates heme degradation. C, RT-PCR for mitochondrial (Mt) ferritin mRNA expression together with densitometric analysis of RT-PCR data. Actin was used as an internal control. 0 h represents control. Data are presented as mean ± S.E. (***, p < 0.001 versus control; **, p < 0.05 versus control; n = 6).