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. 2011 Sep 27;286(45):39417–39430. doi: 10.1074/jbc.M111.295188

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — EMSA on promoter regions in T3SS and T6SS of E. tarda PPD130/91 using DNA binding domains of EsrB, EsrC, PhoB, and full-length Fur. A, EMSA using DNA fragments derived from promoter regions of evpP (401 bp; nt −391 to +11) and evpA (510 bp; nt −507 to +3) in T6SS of E. tarda PPD130/91. B, EMSA using DNA fragments derived from promoter regions of esrC (425 bp; nt −419 to +6), esaB (456 bp; nt −447 to +9), esaM (533 bp; nt −530 to +3), esaR (456 bp; nt −450 to +6), esrA (455 bp; nt −449 to +6), esrB (470 bp; nt −467 to +3), and orf29 (406 bp; nt −400 to + 6) in T3SS of E. tarda PPD130/91. Numbers above each panel represent concentrations (μm) of each protein used in the assay. Labels on the left and right of each panel represent the type of DNA or protein-DNA complexes formed based on their respective molecular weights. For the assay involving Fur, 150 μm FeCl₃ was included in the reaction mixture.